eGFP mRNA-LNP Premade Lipid Nanoparticle Kit
 
eGFP mRNA-LNP is a lipid nanoparticle (LNP) containing Enhanced Green Fluorescent Protein (eGFP) mRNA. LNP can facilitate transfection of encapsulated mRNA into cells or in vivo delivery. After transfection in vitro or endosomal escape in vivo, mRNA can be released and translated into eGFP.
 
This product utilizes the lipid nanoparticle technology for simple and efficient delivery of eGFP mRNA to a variety of mammalian cells, including cell cultures which may typically be prone to difficulties in expressing desired proteins. The LNP’s used are formulated with ionizable lipid, DSPC, cholesterol, and DMG-PEG2000 at optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery.

eGFP is an enhanced version of GFP (green fluorescent protein), originally isolated from Aequorea Victoria, and shows a bright green fluorescence with an emission peak of 509nm. EGFP is a useful marker for tracking gene expression and transfection efficiency in mammalian cultures.  
 
This product is suitable for research of RNA delivery, translation efficiency, cell viability, etc.
 
 
Payload:
eGFP mRNA  (m1Ψ)
Formulation:
Ionizable lipid, DSPC, PEG-2000 DMG, Cholesetrol 
Storage Buffer:
10% Sucrose in PBS
Appearance:
Clear and colorless
Storage:
-20° C or below. We recommend to minimize freeze-thaw cycles to preserve LNPs integrity.
Shipping:
Dry ice
Handling:
Avoid freeze and thaw
Concentration:
~0.1 mg/mL
Stock:                                              
On-demand
 
 Special formulation and other requirements are available up request.   Contact us.
 
For Research Use Only. Not for use in diagnostic procedures.