Firefly Luciferase (FLuc) mRNA
FLuc mRNA encodes a luciferase protein originally sourced from the firefly Photinus pyralis. This luciferase, FLuc, is a common tool in mammalian cell culture, utilized for measuring gene expression and cell viability through bioluminescence emission upon luciferin substrate interaction. This mRNA possesses a Cap1 structure, which is highly efficient in capping. It is fully substituted with N1-methyl-pseudo Uridine to improve expression and decrease immunogenicity. Additionally, the mRNA features a 110A tail in its sequence, resembling a mature mRNA.
The FLuc RNA product is designed to provide researchers with a high-quality and reliable source of this critical tool for their experiments.
- Base Composition: N1-methyl-pseudouridine
- Purity: Passes agarose gel mobility
- Concentration: 1 mg/mL
- Formulation: 1mM Sodium Citrate, pH 6.4
- Storage: ≤ -40°C
- Shipping Method: Dry ice
For research use-only.
Special formulation and other requirements are available upon request.