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NanoGenerator® Flex-S Plus Platform
Automated High-throughput
Discovery & Screening
for Lipid Nanoparticle Formulation

High throughput 96 samples per hour
Small volume output

The NanoGenerator Flex-S Plus is an automated high-throughput formulation screening platform designed for nanoparticle synthesis, including lipid nanoparticles (LNPs), liposomes, and PLGA particles.

Notably, the rapid adoption of LNP technology has underscored the potential of nanomedicines. Scientific advancements have demonstrated that LNPs serve as highly effective drug delivery systems across various therapeutic applications. High-throughput LNP screening is a valuable method for identifying the optimal LNP formulations from a diverse set of factors.

The NanoGenerator Flex-S Plus streamlines this process, enabling a swift transition from screening nanoformulations to clinical evaluation and, ultimately, commercialization. NanoGenerator generates nanoparticles with better uniformity in size and smaller PDI. It is scalable from 0.1mL/sample screening up to 1L (>20L with custom design) large volume GMP production.

System Benefits

High Throughput & Efficiency

Multiple samples (up to 32) per run
Runtime <5min for 4 samples, 96 samples per hour
Scalable and reproducible to large volume production

Automation

Automated workflow
Real-time data monitoring & recording
Electronic batch records

High Yield

Small reagent volume (minimum 100ul) per sample
Save up to 80% of RNA/lipid cost

The Flex-S Plus System facilitates the rapid screening of nanoparticle formulations and early-stage mRNA candidates, offering a substantial increase in project efficiency. With a max throughput of 48/96 experiments an hour, the Flex-S Plus greatly streamlines screening processes. It offers comprehensive automation of complex protocols, enabling users to concentrate on other laboratory duties.

The system also permits experimentation with as little as 20 µl of payload reagent (e.g. mRNA) while providing control over collection volumes. This allows users to optimize the use of valuable materials.

Reproducible
Discovery & Screening

PreciGenome's NanoGenerator platform's precise control of parameters ensures consistent critical quality attributes, such as particle size, throughout the entire development and manufacturing process. This guarantees speed, cost-effectiveness, and reliability at every stage.

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Multi-sample Formulation Screening Example

LNP screening 32sample by S plus, flex s plus consiitancy for LNP thesis screening

32 sample screening (formulation & N:P ratio screening)

Screening factors with the Flex-S Plus include payloads, carrier formulation, total flow rate, flow rate ratio, N:P ratio, Lipid concentration, and payload concentration. Precise control of parameters ensures consistent CQAs, guaranteeing speed, cost-effectiveness, and reliability at every stage of the experiment.

Path from Discovery to Commercialization

NanoGenerator system offers controllable and reproducible mixing conditions, ensuring the accurate synthesis of LNPs through its scalable architecture found in the entire NanoGenerator product line. This architecture facilitates the seamless transfer of crucial process parameters, guaranteeing consistent critical quality attributes (CQAs) as one transitions from discovery and screening, process development to GMP production and commercial manufacturing for a wide range of applications, such as mRNA LNP vaccine development.

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Transferable results from early discovery and screening (Flex-S/Flex-S Plus, 0.1mL) to pre-clinical development (Pro, 200mL), then commercial production (Max: 1L, MAX 40L/H: >20L)

Working Principle

Nanoparticle synthesis via microfluidic mixing has superior control of size, homogeneity, and repeatability to conventional batch synthesis methods. Two streams, aqueous and solvent, meet in a narrow mixing channel, forming nanoparticles with payload encapsulated.

Learn More

Workflow

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Screening factors with the Flex-S Plus include payloads, carrier formulation, total flow rate, flow rate ratio, N:P ratio, and LNP concentration. Precise control of parameters ensures consistent CQAs, guaranteeing speed, cost-effectiveness, and reliability at every stage of the experiment.

Workflow